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Anti-von Willebrand factor (vWF) Antibody, clone IIIE2.34

品牌
Sigma-Aldrich
货号
MABT856
规格纯度
clone IIIE2.34, from mouse
参考价格
1415.25 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-von Willebrand factor (vWF) Antibody, clone IIIE2.34
vWF
产品介绍:

产品说明

一般描述

von Willebrand factor (UniProt: P04275; also known as vWF) is encoded by the VWF (also known as F8VWF) gene (Gene ID:7450) in human. vWF is a multimeric plasma glycoprotein that is synthesized by endothelial cells and plays an important in the maintenance of hemostasis. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form, which is then cleaved into von Willebrand antigen 2 (aa 23-763) and vWF (aa 764-2813). vWF is known to promote adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. vWF also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Mutations in VWF gene are linked to von Willebrand diseases 1-3 that are common hemorrhagic disorders caused by impaired platelet aggregation. Two isoforms of vWF have been described that are produced by alternative splicing.

特异性

Clone IIIE2.34 is a mouse monoclonal antibody that detects human von Willebrand factor. It targets an epitope with in the VWFD3 domain.

免疫原

Protease fragment of VWF binding to heparin sepharose from human plasma.

应用

Research Category
Cell Structure
Anti-von Willebrand factor, clone IIIE2.34, Cat. No. MABT856, is a mouse monoclonal antibody that detects human von Willebrand factor and has been tested for use in Immunocytochemistry and Western blotting.
Western Blotting Analysis: 1 µg/mL from a representative lot detected von Willebrand factor (vWF) in lysates from Human umbilical vein endothelial cells (HUVEC).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected von Willebrand factor (vWF) in Human umbilical vein endothelial cells (HUVEC).

质量

Evaluated by Western Blotting in human platelets cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected von Willebrand factor (vWF) in human platelets cell lysates.

目标描述

~270 kDa observed, 309.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Format: Purified
Protein A purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆IIIE2.34, monoclonal
species reactivityhuman
包装antibody small pack of 25 μg
technique(s)immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_000543
UniProt登记号P04275
Gene Informationhuman ... VWF(7450)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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