产品说明
一般描述
Wiskott-Aldrich Syndrome Protein (WASP)-family Protein Member 2 (known as WASF2/WAVE-2) has a very wide distribution with strong expression in peripheral blood leukocytes. WASF2/WAVE-2 is involved in signaling RTKs and small GTPases. In general WAVE family of proteins have a general function for regulating the actin cytoskeleton in various tissues.
免疫原
His-tagged recombinant protein corresponding to the C-terminus of Human WASF2/WAVE-2.
Epitope: C-terminus
应用
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
This Anti-WASF2/WAVE-2 Antibody, clone ZZ36-12 is validated for use in Western Blotting and Immunocytochemistry for the detection of WASF2/WAVE-2.
Western Blotting Analysis: 1 µg/mL of a representative lot of this antibody detected WASF2/WAVE-2 in 10 µg of K562 cell lysate.
Western Blotting Analysis: A representative lot of this antibody detected WASF2/WAVE-2 in HeLa cell lysate +/- RNAi.
Immunocytochemistry Analysis: A representative lot of this antibody detected WASF2/WAVE-2 in HeLa cells.
质量
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected WASF2/WAVE-2 in 10 µg of HeLa cell lysate.
目标描述
~65 kDa observed.
May run at ~65-80 kDa, which is higher than calculated molecular weight of 54 kDa, due to the proline rich residues in the sequence (Yan, C., et al. (2003) EMBO J. 22(14): 3602–3612).
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | ZZ36-12, monoclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
technique(s) | immunocytochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NP_001188333 |
UniProt登记号 | Q9Y6W5 |
运输 | wet ice |
Gene Information | human ... WASF2(10163) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich