产品说明
一般描述
The gene XIRP1 (xin actin-binding repeat-containing protein-1) is mapped to human chromosome 3p22.2. It belongs to XIRP family. The protein is expressed during early developmental stages of cardiac and skeletal muscles. In adults, XIRP1 is mainly present in the intercalated discs of cardiomyocytes and the myotendinous junctions of skeletal muscle cells. The protein contains unique 16 amino acid repeats. Fluorescent tagging of the protein showed targeting to F-actin containing structures.
免疫原
Xin actin-binding repeat-containing protein 1 recombinant protein epitope signature tag (PrEST)
应用
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.
生化/生理作用
Down-regulation of xin actin-binding repeat-containing protein-1 (XIRP1) in cultured chicken embryos results in abnormal development of the heart. XIRP1 is up-regulated in injured mouse muscle. The unique amino acid repeats in XIRP1 are responsible for binding and stabilization of actin-based cytoskeleton. XIRP1 interacts with phosphoglucomutase-like protein 5 (PGM5) and filamin C. All three together are present at intercalated discs of cardiac muscle and myotendinous junctions of skeletal muscle. XIRP1 also associates with the EVH1 (Ena/Vasp homology) domain proteins MENA (Protein enabled homolog) and VASP (Vasodilator-stimulated phosphoprotein). In adult heart, XIRP1 and MENA/VASP localizes with filamin C in intercalated discs.
特点和优势
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
联系
Corresponding Antigen APREST74653.
外形
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
法律信息
Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
人类蛋白质图谱编号 | HPA018458 Human Protein Atlas characterization data |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
偶联物 | unconjugated |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
产品线 | Prestige Antibodies® Powered by Atlas Antibodies |
形式 | buffered aqueous glycerol solution |
species reactivity | human |
包装 | antibody small pack of 25 μL |
增强验证 | independent orthogonal RNAseq Learn more about Antibody Enhanced Validation |
technique(s) | immunohistochemistry: 1:200-1:500 |
免疫原序列 | RVSTEVAQLKEQTLARLLDIEEAVHKALSSMSSLQPEASARGHFQGPPKDHSAHKISVTVSSSARPSGSGQEVGGQTAVKNQAKVECHTEAQSQVKIRNHT |
UniProt登记号 | Q702N8 |
运输 | wet ice |
储存温度 | −20℃ |
Gene Information | human ... XIRP1(165904) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich