产品说明
一般描述
Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.
外形
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
重悬
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 month. For extended storage, it is recommended to store at -20℃.
基本信息
NACRES | NA.32 |
产品性质
生物来源 | human |
重组 | expressed in HEK 293 cells |
标签 | 6-His tagged (C-terminus) |
测定 | ≥95% (SDS-PAGE) |
形式 | lyophilized |
分子量 | calculated mol wt 26.7 kDa observed mol wt 43-60 kDa (DTT-reduced.Protein migrates due to glycosylation. Gly 24 is the predicted N-terminal.) |
包装 | pkg of 10 μg pkg of 50 μg |
杂质 | <1 EU/μg endotoxin (LAL test) |
UniProt登记号 | P42081 |
运输 | wet ice |
储存温度 | −20℃ |
Gene Information | human ... CD86(942) |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich