产品说明
一般描述
β-Lactamase (β-lactamase, βL, EC 3.5.2.6) is an enzyme first identified in Escherichia coli and has been described as penicillinase. A number of βLs have since been identified from various bacteria. β-Lactamases specifically hydrolyze β-lactam rings present in antibiotics such as penicillin, cephalosporins, monobactam, and carbapenem, and confer resistance against these antibiotics.
应用
Beta-Lactamase Inhibitor Screening Kit has been used in enzymatic assays.
特点和优势
Compatible with high-throughput handling systems.
适用性
适于筛选β-内酰胺酶抑制剂。
原理
The β-Lactamase Inhibitor Screening Kit is a rapid, simple and sensitive assay that is suitable for high throughput screening of β-Lactamase inhibitors. βL activity is measured by hydrolyzing a chromogenic cephalosporin called nitrocefin, producing a colorimetric product (A490), proportional to the enzymatic activity present.
产品性质
detection method | colorimetric |
储存温度 | −20℃ |
组份列表
组份不可单独销售
货号 | 组份 |
β-Lactamase Assay Buffer | |
Nitrocefin, in DMSO | |
β-Lactamase | |
Inhibitor Control, Clavulanic acid |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H317 - H334 |
预防措施声明 | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
危险分类 | Resp. Sens. 1 - Skin Sens. 1 |
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
Sigma-Aldrich