Calprotectin, human recombinant

产品说明

一般描述

This recombinant human calprotectin is a heterodimer composed of tagged versions of both S100A8 and S100A9, a 6xHis-SUMO tag is added to the N-term of S100A9, a 6xHIS tag is added to the N-term of S100A8. This tagged version was found to have enhanced stability as compared to untagged versions of this protein. Removal of the tags can be achieved using HRV-3C protease (SAE0045 or SAE0110).

应用

Fecal Calprotectin is often used as a biomarker to identify inflammation in gastrointestinal disease¹. This product is a recombinant version of the human protein found endogenously.

生化/生理作用

Calprotectin is a calcium and zinc binding protein that belong to the S100 family, it is heterodimer protein composed of S100A8 and S100A91. The protein is expressed in myeloid cells and in circulating monocytes and neutrophils, where it accounts for 60% of the cytosolic protein content. Calprotectin was found to play major role in inflammation and immune response by activation of NF-kB pathway via TLR4 and AGER2, and its antimicrobial activity possibly through chelating of Zn3. High concentration of calprotectin is found and serves as a biomarker in rheumatoid, cystic fibrosis, inflammatory bowel disease and in Crohn′s disease4. A meta-analysis also identified calprotectin as a potential biomarker for COVID-195.

外形

The product is lyophilized from a sterile 0.2 mm filtered solution containing 12.5 mM Tris Cl pH 8.0, 150 mM NaCl, 1mM DTT.

制备说明

Reconstitute lyophilized vial in DMEM or in PBS to a concentration up to 500 mg/mL, spin down to collect material. Reconstituted product should be stored in working aliquots at –20℃. Do not store in frost-free freezer. Avoid freeze-thaw cycles.

产品性质

安全信息

象形图
警示用语：	Warning
危险声明	H319
预防措施声明	P264 - P280 - P305 + P351 + P338 - P337 + P313
危险分类	Eye Irrit. 2
储存分类代码	11 - Combustible Solids
WGK	WGK 2