产品说明
一般描述
Programmed cell death 1 ligand 1 (PD-L1), which is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules. PD-L1 is a cell surface immunoglobulin with two Ig-like domains within the extracellular region and a short cytoplasmic domain. The protein is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. Recombinant mouse CD274 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
生化/生理作用
Programmed cell death 1 ligand 1 (PD-L1) is involved in the regulation of cellular and humoral immune responses. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after lipopolysaccharide (LPS) and interferon-γ (IFNG) activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 is involved in the co-stimulatory signal, essential for T-cell proliferation and production of interleukin-10 (IL10) and IFNG, in an interleukin-2-dependent and a programmed cell death 1 (PDCD1)-independent manner.
外形
1 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl and 10% glycerol.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | mouse |
| 重组 | expressed in E. coli |
| 测定 | ≥85% (SDS-PAGE) |
| 形式 | liquid |
| 分子量 | 27.6 kDa (246 aa, 19-239 aa + His tag) |
| 包装 | pkg of 100 μg |
| 浓度 | 1 mg/mL |
| 杂质 | <1 EU/μg endotoxin (LAL test) |
| UniProt登记号 | Q9EP73 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | mouse ... CD274(60533) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
