产品说明
一般描述
Human ceramide kinase (CERK) (GenBank Accession No. NM_022766), 1-537 (end) with C-terminal Flag-tag, MW= 60 kDa, expressed in Baculovirus expression system.
Ceramide kinase (CERK) is a 537-amino acid protein. It has a catalytic region, a putative amino-myristoylation site in its amino terminal region and a pleckstrin homology domain. The gene encoding CERK is localized on human chromosome 22q13.31.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生化/生理作用
Ceramide kinase (CERK) is an enzyme involved in sphingolipid metabolism and in the synthesis of ceramide-1-phosphate. It associates with calmodulin and at the time of Fc receptor-mediated phagocytosis, it aggregates in lipid rafts. CERK may have a role in calcium signaling. The protein functions in the phagocytosis of polymorphonuclear leukocytes and degranulation of mast cells.
单位定义
One unit is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37℃.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected Sf9 cells |
| 测定 | ≥40% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 60 kDa |
| 包装 | pkg of 10 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 浓度 | 0.4 mg/mL |
| NCBI登记号 | NM_022766 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... CERK(64781) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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