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位置:首页 > 品牌 > Sigma-Aldrich > ChemiSCREEN Membrane Preparation Recombinant Human CysLT 1 Cysteinyl Leukotriene Receptor

ChemiSCREEN Membrane Preparation Recombinant Human CysLT 1 Cysteinyl Leukotriene Receptor

品牌
Sigma-Aldrich
货号
HTS061M
包装型号
规格纯度
参考价格
10463.8 *本价格含增值税费
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数量
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产品名称:
ChemiSCREEN Membrane Preparation Recombinant Human CysLT 1 Cysteinyl Leukotriene Receptor
产品介绍:

产品说明

一般描述

The cysteinyl leukotrienes, leukotriene C4, leukotriene D4 and leukotriene E4, are arachidonic acid derivatives modified by glutathione, Cys-Gly or Cys. Activated mast cells release cysteinyl leukotrienes, which cause smooth muscle contraction, airway constriction, and vascular permeability. The biological effects of the cysteinyl leukotrienes are mediated by two GPCRs, CysLT1 and CysLT2. A CysLT1 selective antagonist, montelukast, is used clinically in the treatment of asthma (Brink et al., 2003; Evans, 2002). Chemicon′s CysLT1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CysLT1 interactions and its ligands. The membrane preparations exhibit a Kd of 0.97nM for [33H]-Leukotriene D4 (LTD4). With 10.0ug/well CysLT1 membrane prep and 1.5 nM [33H]-Leukotriene D4, a greater than 3-fold signal-to-background ratio was obtained.
Full-length human CYSLTR1 cDNA

应用

Human CysLT1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Radioligand binding assay and GTPγS binding

生化/生理作用

  • GPCR Class: A
  • Protein Target: CysLT1
  • Target Sub-Family: Leukotriene

产品规格

Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50 mM Tris, pH 7.4. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

Binding buffer: 50 mM Tris, pH7.4, 20 mM CaCl2, 25 mM MgCl2, 10 mM Glycine, 10 mM L-Cysteine, filtered and stored at 4℃.

Radioligand: [3H] LTD4 (Perkin Elmer#: NET-101)

Wash Buffer: 50 mM Tris, pH 7.4, filtered and stored at 4℃.

外形

One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 3-fold signal:background with 3H-labeled LTD4 at 1.5 nM.

Liquid in packaging buffer: 50 mM

储存及稳定性

Maintain frozen at -70℃ for up to 2 years. Do not freeze and thaw.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

基本信息

eCl@ss32161000

产品性质

质量水平100
生物来源human
重组expressed in Chem-1 cells
manufacturer/tradenameChemiScreen
Chemicon®
technique(s)ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable
NCBI登记号NM_006639.2
UniProt登记号Q9Y271

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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