ColorWheel^® Antibody-Ready Alpha Fluor 488 Dye

产品说明

一般描述

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Alexa Fluor^® 488 (AF488)-NHS Ester (Succinimidyl Ester) is a water soluble, bright green fluorescent dye that exhibits an excitation max at 499 nm and an emission max at 520 nm with an extinction coefficient of 73,000 cm-1 M-1. It is suitable for use over a wide range of pH (4 to 10). It can be used with the 488 nm Argon laser and its spectral properties are identical to Alexa Fluor^® 488. The NHS ester is the most convenient amine-reactive form for conjugating it to proteins and antibodies and can be used for stable signal generation in Flow cytometry. It displays high photostability and brighter fluorescence.

应用

Flow Cytometry: Excitation: 499 nm, Emission: 520 nm

相容性

Requires ColorWheel^® Antibody (Sold Separately)

外形

CWDS-AF488-25UL Set Components

CWD-AF488-25UL - ColorWheel^® Antibody-Ready Alpha Fluor 488 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL -ColorWheel^® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

重悬

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质