产品说明
一般描述
Members of the Dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumor suppressor gene and a marker for tumor blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear β-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of β-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.
外形
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
重悬
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 month. For extended storage, it is recommended to store at -20℃.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in HEK 293 cells |
| 标签 | 6-His tagged (C-terminus) |
| 测定 | ≥95% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 分子量 | calculated mol wt 37.6 kDa observed mol wt 50-60 kDa (DTT-reduced. Protein migrates due to glycosylation. Pro 23 is the predicted N-terminus.) |
| 包装 | pkg of 50 μg |
| 杂质 | <1 EU/μg endotoxin (LAL test) |
| UniProt登记号 | Q9UBP4 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... DKK3(27122) |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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