您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > EGFR (T790M L858R) Active human

EGFR (T790M L858R) Active human

品牌
Sigma-Aldrich
货号
SRP0242
规格纯度
recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
参考价格
6182.85 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
EGFR (T790M L858R) Active human
ERBB1
proto-oncogene c-ErbB-1
PIG61
HER1
Epidermal growth factor receptor
产品介绍:

产品说明

一般描述

Epidermal growth factor receptor (EGFR) can be activated by binding EGF or at least six other structurally related protein ligands, including transforming growth factor-α (TGF-α), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), amphiregulin, epiregulin and epigen. Human EGFR T790M L858R (GenBank Accession No. NM_005228), (amino acids 668-1210) with N-terminal GST tag, MW = 88kDa, expressed in baculovirus expression system.
The EGFR gene is mapped to human chromosome 7p11.2 and encodes a 170 kDa receptor protein belonging to the HER (Receptor tyrosine-protein kinase erbB-4) family of receptor tyrosine kinases. Egfr is localized to epithelial cell surface.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化/生理作用

Epidermal growth factor receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. The receptor undergoes dimerization and phosphorylation of tyrosine kinase. Upon activation by ligands, epidermal growth factor receptor (EGFR, ErbB1) initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers. Mutation in the EGFR gene is associated with the risk of tumor development including glioma, colorectal adenoma, colorectal, breast and non-small cell lung cancer.

单位定义

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30℃.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRESNA.32

产品性质

生物来源human
重组expressed in baculovirus infected insect cells
测定≥70% (SDS-PAGE)
形式aqueous solution
分子量 88 kDa
包装pkg of 10 μg
储存条件avoid repeated freeze/thaw cycles
浓度>0.02 mg/mL
NCBI登记号NM_005228
UniProt登记号P00533
运输dry ice
储存温度−70℃
Gene Informationhuman ... EGFR(1956)

Sigma-Aldrich

推荐产品