产品说明
生化/生理作用
Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′ (5 ). A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an α-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.
外形
Clear and colorless frozen liquid solution
制备说明
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
基本信息
NACRES | NA.26 |
产品性质
生物来源 | Saccharomyces cerevisiae |
重组 | expressed in E. coli |
测定 | ≥80% (SDS-PAGE) |
形式 | frozen liquid |
分子量 | ~18.6 kDa |
包装 | pkg of 10 μg |
储存条件 | avoid repeated freeze/thaw cycles |
浓度 | 300 μg/mL |
technique(s) | electrophoretic mobility shift assay: suitable |
颜色 | colorless to clear |
NCBI登记号 | NM_001184062 |
UniProt登记号 | P04386 |
运输 | dry ice |
储存温度 | −70℃ |
Gene Information | Saccharomyces cerevisiae ... GAL4(855828) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich