产品说明
一般描述
Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics.
特点和优势
适用性
The kit is suitable for high throughput inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.
原理
The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.
产品性质
质量水平 | 100 |
detection method | colorimetric |
相关疾病 | hematological disorder, anemia; cancer |
储存温度 | −20℃ |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H341 |
预防措施声明 | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
危险分类 | Muta. 2 |
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
Sigma-Aldrich