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Glutathione S-transferase Assay Kit

品牌
Sigma-Aldrich
货号
MAK453
规格纯度
Sufficient for 100 colorimetric tests
参考价格
3989.77 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Glutathione S-transferase Assay Kit
产品介绍:

产品说明

一般描述

Glutathione transferase (GST) is a multifunctional enzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compounds electrophilic and/or hydrophobic sites.

特点和优势

  • Fast and sensitive. Linear detection range (20 μL sample): 2 to 80 U/L for 10 min reaction at 25 ℃.
  • Compatible with high-throughput handling systems.

适用性

The kit is suitable for Glutathione S-transferase activity determination in cell lysates, tissues, etc.

原理

The Glutathione S-transferase Assay Kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1- chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at A340 nm. The rate of increase in absorbance at A340 nm is directly proportional to the GST activity in the sample.

产品性质

质量水平100
detection methodcolorimetric
相关疾病neurological disorders, asthma; pulmonary disorders, diabetes; endocrinological disorders; cancer
储存温度−20℃

安全信息

象形图GHS07
警示用语:Warning
危险声明H317 - H319 - H412
预防措施声明P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
危险分类Aquatic Chronic 3 - Eye Irrit. 2 - Skin Sens. 1
储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)188.6 °F
闪点(C)87 ℃

Sigma-Aldrich

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