产品说明
一般描述
Complex of human HDAC3, GenBank Accession No. NM_003883, full length with C-terminal His tag, MW = 49.7 kDa. Human NCOR2, amino acid 395-489, GenBank Accession No. NM_006312, N-terminal ST tag, MW = 37.6 kDa, co-expressed in baculovirus expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37℃.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 20 mM glutathione and 10% glycerol.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected insect cells |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 37.6 kDa (NCOR2) 49.7 kDa (HDAC3) |
| 包装 | pkg of 50 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_003883 |
| UniProt登记号 | O15379 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... HDAC3(8841) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
