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ICAM2 human

品牌
Sigma-Aldrich
货号
5107
规格纯度
recombinant, expressed in E. coli, 0.5 mg protein/mL
参考价格
3485.44 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
ICAM2 human
产品介绍:

产品说明

应用

Coating a plate well (6 well plate) with this recombinant ICAM2 protein in neuronal cell specific medium at 1-10 μg / well allows for 1) human lymphocyte cell / receptor interaction studies in vitro and 2) use as a culture matrix protein for anti-tumor immuno-response studies in vitro.

Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
1. Thaw ICAM2 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly.
Note: Use 1 ml PBS per well in a 6-well plate.
2. Add 1 - 10 μg protein to each well and incubate at 2-10 ℃ overnight.
3. After incubation, aspirate remaining material.
4. Plates are ready for use. They may also be stored at 2-8 ℃ damp or air dried if sterility is maintained.

序列

MASMTGGQQMGRGHHHHHHGNLYFQGGEFELKVFEVHVRPKKLAVEPKGSLEVNCSTTCNQPEVGGLETSLDKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQ

制备说明

The full-length extracellular domain of human ICAM2 gene (25-223 aa) was constructed with 29 N-terminal T7/HIS-tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.

基本信息

NACRESNA.75

产品性质

生物来源human
重组expressed in E. coli
描述0.1 mg recombinant human ICAM2 in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
无菌性Filtered sterilized solution
测定≥90% (SDS-PAGE)
形式liquid
包装pkg of 100 μg
浓度0.5 mg protein/mL
登记号NP_000864
UniProt登记号P13598
储存温度−20℃
Gene Informationhuman ... ICAM2(3384)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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