产品说明
一般描述
Mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5) is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines. This 471 amino acid protein is widely expressed in tissues. The gene encoding MAPKAPK5 is localized on human chromosome 12q24.12-q24.13.
生化/生理作用
Mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5) is activated through its phosphorylation by MAP kinases including MAPK1, MAPK14, and MAPK11. It is activated in HeLa cells in response to cellular stress and proinflammatory cytokines. MAPKAPK5 activity is regulated by p38-α and p38-β both in vitro and in vivo, and thr-182 is the regulatory phosphorylation site of MAPKAPK5. In vitro, the protein phosphorylates heat shock protein HSP27 at its physiologically relevant sites. It may have a role in actin polymerization, cell motility and tumor suppression.
外形
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
制备说明
after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
产品性质
| 重组 | expressed in baculovirus infected Sf9 cells |
| 产品线 | PRECISIO® Kinase |
| 测定 | ≥70% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| specific activity | 146-198 nmol/min·mg |
| 分子量 | ~79 kDa |
| NCBI登记号 | NM_003668 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... MAPKAPK5(8550) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
Sigma-Aldrich
m.cnreagent.com
