产品说明
一般描述
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
储存及稳定性
Avoid freeze / thaw cycles. Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.
产品性质
生物来源 | rabbit |
质量水平 | 100 |
偶联物 | unconjugated |
antibody product type | primary antibodies |
克隆 | monoclonal |
形式 | liquid |
species reactivity | SARS virus |
technique(s) | ELISA: 1:1000-1:5000 flow cytometry: 1:50-1:200 immunofluorescence: 1:50-1:200 immunohistochemistry: 1:50-1:200 immunoprecipitation (IP): 1:50-1:200 western blot: 1:1000-1:5000 |
同位素/亚型 | IgG |
NCBI登记号 | P59595 |
运输 | wet ice |
储存温度 | −20℃ |
Gene Information | SARS virus ... N(1489678) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich