产品说明
一般描述
Monoclonal Anti-CDA (mouse IgG2a isotype) is derived from the hybridoma CDA-40 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human CDA conjugated to KLH. CDA (Cytidine deaminase) also known as cytidine aminohydrolase or CDD. The enzyme is active only as an intact tetramer, since three different monomers concur to the formation of each of the four active sites.
免疫原
synthetic peptide corresponding to an internal sequence of human CDA .
应用
Monoclonal Anti-CDA antibody may be used in several immunochemical techniques including:
生化/生理作用
Cytidine deaminase (CDA) is an intracellular enzyme of the pyrimidine salvage pathways. It catalyzes that deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. In addition, It also catalyzes the deamination of cytosine nucleoside analogues such as ARA-C (cytosine arabinoside) and 5-AZA-CdR (5-aza-2′-deoxycytidine) used in chemotherapy, leading to the loss of their pharmacological activity. CDA inhibitors such as tetrahydrouridine (THU) are interesting therapeutically for use in combination with chemotherapy drugs in patients with leukemia, to enhance drugs activity.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | CDA-40, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~16 kDa |
| species reactivity | human |
| 浓度 | ~1 mg/mL |
| technique(s) | flow cytometry: 5-10 μg/test using using HeLa cells. immunoblotting: 0.5-1 μg/mL using whole extracts of HeLa cells. immunofluorescence: 4-8 μg/mL using using HeLa cells. |
| 同位素/亚型 | IgG2a |
| UniProt登记号 | P32320 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... CDA(978) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
