Monoclonal Anti-CHD1 antibody produced in rat

产品名称：

产品介绍：

产品说明

一般描述

Monoclonal Anti-CHD1 (rat IgG2a isotype) is derived from the hybridoma 2F11H5 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with a synthetic peptide. Chromodomain-helicase-DNA-binding protein 1 (CHD1) is an ATP-dependent chromatin-remodeling factor expressed majorly in cerebellum and basal ganglia. CHD1 gene is mapped to human chromosome 5q15-q21.1. CHD1 comprises two chromodomains.

特异性

Monoclonal Anti-CHD1 recognizes human CHD1.

免疫原

synthetic peptide corresponding to a sequence at the C-terminal region of human CHD1.

应用

Monoclonal Anti-CHD1 antibody produced in rat may be used in:

immunofluorescence
flow cytometry
immunoblotting
immunohistochemistry

生化/生理作用

Chromodomain-helicase-DNA-binding protein 1 (CHD1) interacts with the trimethylated lysine residues of histones and alters access of the transcriptional apparatus to its chromosomal DNA template. CHD1 is required for maintenance of open chromatin and pluripotency in embryonic stem cells. Missense mutation in the CHD1 gene is implicated in the Pilarowski-Bjornsson syndrome as well as in speech delay and facial dismorphism.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2–8 ℃ for up to one month. For extended storage,freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

产品性质

质量水平	200
生物来源	rat
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	2F11H5, monoclonal
形式	buffered aqueous solution
species reactivity	human
浓度	~1 mg/mL
technique(s)	flow cytometry: 5-10 μg/test using using HeLa cells immunofluorescence: 20 μg/mL using using human HeLa cells.
同位素/亚型	IgG2a
UniProt登记号	O14646
运输	dry ice
储存温度	−20℃
Gene Information	human ... CHD1(1105)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable