NAD+/NADH Assay Kit

产品说明

一般描述

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue.

特点和优势

• Detection limit of 0.02 μM and linearity up to 1 μM NAD+/NADH in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 minutes.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the determination of NAD and NADH and the evaluation of drug effects on NAD/NADH metabolism in cells, tissue extracts etc.

原理

The NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.

产品性质

安全信息

象形图
警示用语：	Warning
危险声明	H290
预防措施声明	P234 - P390
危险分类	Met. Corr. 1
储存分类代码	8A - Combustible, corrosive hazardous materials
WGK	WGK 3