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PARP6 Active human

品牌
Sigma-Aldrich
货号
SRP0195
规格纯度
recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
参考价格
9062.7 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
PARP6 Active human
Poly (ADP-ribose) Polymerase 6
pART17
MGC131971
产品介绍:

产品说明

一般描述

PARP6 (poly(ADP-ribose) polymerase family member 6) gene is localized to human chromosome 15q23. It is a member of the family of multifunctional nuclear proteases known as PARPs, which contains 17 members and is further classified into three subtypes dependent on the catalytic domain structure. PARP6 is a type of mono(ADP-ribosyl) transferase on the basis that its catalytic domain has a significantly shorter nicotinamide-ribose-binding site than PARP1. This subfamily contains nine members including PARP6. PARP6 is composed of 630 residues and has a molecular weight of 71kDa.
Human PARP6 (GenBank Accession No. NM_020214), full length with N-terminal GST tag, MW = 98kDa, expressed in a Baculovirus infected Sf9 cell expression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化/生理作用

PARP6 (poly(ADP-ribose) polymerase family member 6) negatively controls cell cycle, as its ectopic expression in HeLa cells leads to suppression of cell growth. It functions as a tumor suppressor in CRC (colorectal cancer), and its expression is associated with good prognosis. The ectopic expression of PARP6 results in suppressed expression of the oncogene survivin. In CRC PAPR6 prevents colony formation, invasion, migration, and cell proliferation.

单位定义

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRESNA.32

产品性质

生物来源human
重组expressed in baculovirus infected insect cells
测定≥70% (SDS-PAGE)
形式aqueous solution
分子量 98 kDa
包装pkg of 10 μg
浓度>0.02 mg/mL
NCBI登记号NM_020214
UniProt登记号Q2NL67
运输dry ice
储存温度−70℃
Gene Informationhuman ... PARP6(56965)

Sigma-Aldrich

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