产品说明
生化/生理作用
PDHK1 or Pyruvate Dehydrogenase Kinase 1 is a member of the PDHK family that phosphorylate and inactivate the Pyruvate Dehydrogenase (PDH). PDH is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDHK1 is an important regulator of PDH in clonal beta-cells and the activity of PDHK1 and PDH are important for efficient metabolic coupling. Maintaining low PDHK1 expression/activity and keeping PDH in a dephosphorylated and active state, is important for beta-cells to achieve the metabolic flux rates necessary for maximal glucose-stimulated insulin secretion.
外形
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
制备说明
after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected Sf9 cells |
| 产品线 | PRECISIO® Kinase |
| 测定 | ≥70% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| specific activity | 1.4-2 nmol/min·mg |
| 分子量 | ~70 kDa |
| NCBI登记号 | NM_002610 |
| application(s) | cell analysis |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... PDK1(5163) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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