产品说明
一般描述
Peptidyl Arginine Deiminases (PADs) perform post-translational deiminations of proteins. PADs are calcium-dependent enzymes that catalyze the conversion of
Protein--[
This deimination provides another level of regulating protein function.
There are five mammalian PADs sub-types, which differ in substrate specificity and tissue distribution. PAD enzymes are highly homologous, with 50–60% sequence similarity. PADenzymes play important roles in gene regulation by citrullination of arginine residues on histones H3, H2A, and H4. Overexpression of these enzymes has been found in several diseases such as rheumatoid arthritis, Alzheimer′s disease, multiple sclerosis, lupus, Parkinson′s disease, and cancer. PAD2 is widely expressed in the brain, secretory glands, and skeletal muscles. The enzyme was found to be responsible for hyper-citrullination of myelin basic protein, which is suspected to lead to multiple sclerosis. The product is supplied in 20 mM Tris, pH 7.5, 200 mM NaCl, 10% (w/v) glycerol, 10 mM 2-mercaptoethanol, 1 mM EDTA, 1 mM DTT, 10 mM CaCl2, 10 mM arginine, 30 mM maltose, and 0.6 mg/ml dodecyl−β
单位定义
One unit will produce 1.0 μmole of N-a-benzoylcitrulline ethyl ester from BAEE per hr at 55 ℃ at pH 7.2.
外形
Supplied as a buffered aqueous solution containing Trizma, NaCl, glycerol, mercaptoethanol, EDTA, DTT, CaCl2, arginine, maltose, and dodecyl-β-D-maltoside.
制备说明
Contains a maltose-binding protein fusion tag.
基本信息
酶学委员会(EC)编号 | 3.5.3.15 ( BRENDA | IUBMB ) |
产品性质
质量水平 | 200 |
重组 | expressed in E. coli |
specific activity | ≥40 units/mg protein |
运输 | dry ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich