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位置:首页 > 品牌 > Sigma-Aldrich > PhosphoDetect Anti-p53 (pSer15) (Ab-3) Rabbit pAb

PhosphoDetect Anti-p53 (pSer15) (Ab-3) Rabbit pAb

品牌
Sigma-Aldrich
货号
PC386
规格纯度
liquid, Calbiochem®
参考价格
6190.47 *本价格含增值税费
促销
服务
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数量
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产品名称:
PhosphoDetect Anti-p53 (pSer15) (Ab-3) Rabbit pAb
产品介绍:

产品说明

一般描述

This PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser15.


Recognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.

免疫原

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH

包装

50 μL in Plastic ampoule

应用


Immunoblotting (1:1000)
Immunoprecipitation (1:200)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

分析说明

Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents

其他说明

Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式liquid
不包含 preservative
species reactivityhuman, mouse, rat
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
同位素/亚型IgG
运输wet ice
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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