产品说明
一般描述
This PhosphoDetect
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser15.
Recognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
包装
50 μL in Plastic ampoule
应用
Immunoblotting (1:1000)
Immunoprecipitation (1:200)
警告
Toxicity: Standard Handling (A)
外形
In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
重悬
Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
分析说明
Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
其他说明
Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | liquid |
不包含 | preservative |
species reactivity | human, mouse, rat |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze |
同位素/亚型 | IgG |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
Sigma-Aldrich