产品说明
应用
Useful for Western blot and kinase assays.
生化/生理作用
Recombinant full-length human PI3K (p85alpha) was expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. p85α modulates the interaction between PI3K and platelet-derived growth factor receptor (1). Furthermore, estrogen receptor isoform ER-alpha binds in a ligand-dependent manner to the p85-alpha regulatory subunit of PI3K. Stimulation with estrogen increases ER-alpha-associated PI3K activity, leading to the activation of protein kinase B/AKT and endothelial nitric oxide synthase (eNOS) (2).
重悬
Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
产品性质
重组 | expressed in baculovirus infected Sf9 cells |
测定 | 70-95% (SDS-PAGE) |
形式 | frozen liquid |
分子量 | ~86 kDa |
浓度 | 0.2 μg/μL |
登记号 | NM_181523 |
运输 | dry ice |
储存温度 | −70℃ |
Gene Information | human ... PI3K (5295) |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H315 - H319 - H360D |
预防措施声明 | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
危险分类 | Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 |
储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich