产品说明
应用
Useful for kinase assay.
生化/生理作用
Recombinant full-length human PIP4K2B was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP4K2B (phosphatidylinositol-5-phosphate 4-kinase, type II, beta) catalyzes the phosphorylation of phospha-tidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. PIP4K2B is a member of the phosphatidylinositol-5-phosphate 4-kinase family. PIP5K2B interacts specifically with the juxtamembrane region of the p55 TNF receptor and that PIP5K2B activity is increased in mammalian cells by treatment with TNF-alpha (1). PIP5P4KB plays a role in determining insulin sensitivity and adiposity (2).
重悬
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
产品性质
| 重组 | expressed in baculovirus infected Sf9 cells |
| 测定 | 70-95% (SDS-PAGE) |
| 形式 | frozen liquid |
| specific activity | 4500-6000 nmol/min·mg (ADP-Glo) |
| 分子量 | ~73 kDa |
| 浓度 | 0.1 μg/μL |
| 登记号 | NM_003559 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... PIP4K2B(8396) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
