产品说明
一般描述
Also available: Cell Comb
Introduction
Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade.
The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrix
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Test Principle:
The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, a 24-well tissue culture plate with 12 cell culture inserts. The inserts contain an 8 μm pore size polycarbonate membrane, over which a thin layer of ECMatrixTM is dried. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane.
Application:
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
包装
1 plate in 12 wells
12 wells
应用
Research Category
Cell Structure
Detection of migrating cells is via crystal violet stain.
组分
Invasion Chamber: (Part No. 70019) One 24-well plate with 12 ECMatrixTM layered cell culture inserts.
Cell Stain: (Part No. 20294) One 10 mL bottle.
Cotton Swabs: (Part No. 10202) 24 each.
Forceps: (Part No. 10203) One each.
储存及稳定性
Store kit materials at 2-8°C for up to 6 months. Do not freeze.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32161000 |
NACRES | NA.84 |
产品性质
质量水平 | 100 |
species reactivity | vertebrates |
manufacturer/tradename | Chemicon® QCM |
technique(s) | cell based assay: suitable |
detection method | colorimetric |
运输 | wet ice |
安全信息
储存分类代码 | 10-13 - German Storage Class 10 to 13 |
Sigma-Aldrich