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QCM ECMatrix Cell Invasion Assay, 24-well (8 μm), colorimetric

品牌
Sigma-Aldrich
货号
ECM550
规格纯度
The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
参考价格
7280.59 *本价格含增值税费
促销
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数量
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产品名称:
QCM ECMatrix Cell Invasion Assay, 24-well (8 μm), colorimetric
产品介绍:

产品说明

一般描述

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Introduction
Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade.

The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrix, a reconstituted basement membrane matrix of proteins derived from the Engelbreth Holm-Swarm (EHS) mouse tumor. We examined the kit′s performance using human fibrosarcoma (HT-1080) and non-invasive fibroblasts (NIH3T3).

The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.

The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

Test Principle:

The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, a 24-well tissue culture plate with 12 cell culture inserts. The inserts contain an 8 μm pore size polycarbonate membrane, over which a thin layer of ECMatrixTM is dried. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane.

Application:

The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.

The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

包装

1 plate in 12 wells
12 wells

应用

Research Category
Cell Structure
Detection of migrating cells is via crystal violet stain.

组分

Invasion Chamber: (Part No. 70019) One 24-well plate with 12 ECMatrixTM layered cell culture inserts.

Cell Stain: (Part No. 20294) One 10 mL bottle.

Cotton Swabs: (Part No. 10202) 24 each.

Forceps: (Part No. 10203) One each.

储存及稳定性

Store kit materials at 2-8°C for up to 6 months. Do not freeze.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32161000
NACRESNA.84

产品性质

质量水平100
species reactivityvertebrates
manufacturer/tradenameChemicon®
QCM
technique(s)cell based assay: suitable
detection methodcolorimetric
运输wet ice

安全信息

储存分类代码10-13 - German Storage Class 10 to 13

Sigma-Aldrich

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