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Set7/Set9 human

品牌
Sigma-Aldrich
货号
SRP0149
规格纯度
recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
参考价格
8831.52 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Set7/Set9 human
KMT7
SETD7
SET domain containing (lysine methyltransferase) 7
SET7/9
Lysine N-methyltransferase 7
产品介绍:

产品说明

一般描述

Human recombinant SET7/9, amino acids 52-end, with N-terminal GST tag, GenBank Accession No. NM_030648, expressed in a E. coli expression system. MW = 61 kDa,
SET7/9 also known as KMT7/SET domain containing lysine methyltransferase 7 (SETD7), is encoded by the gene mapped to human chromosome 4q31.1. The encoded protein belongs to SET domain-containing protein family.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化/生理作用

SET7/9 acts as a histone methyltransferase and catalyzes monomethylation of histone H3 at lysine 4 (H3K4me1) in vitro. Down-regulation of SET7/9 expression affects human embryonic stem cell differentiation. The encoded protein is implicated in the regulation of proteins and transcription factor activity. It also stimulates promoters of methylation-dependent co-recruitment through non-histone protein methylation. Mutation in the gene encoding SET7/9 is associated with serum lycopene concentrations. Elevated expression of the gene is associated with progression of hepatocellular carcinoma (HCC).

单位定义

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37℃.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRESNA.32

产品性质

生物来源human
重组expressed in E. coli
测定≥70% (SDS-PAGE)
形式aqueous solution
分子量 61 kDa
包装pkg of 100 μg
储存条件avoid repeated freeze/thaw cycles
浓度>0.02 mg/mL
NCBI登记号NM_030648
UniProt登记号Q8WTS6
运输dry ice
储存温度−70℃
Gene Informationhuman ... SETD7(80854)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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