产品说明
一般描述
SMAD4 is a member of the SMAD family and mediates signaling by the transforming growth factor-beta (TGFβ) superfamily and related ligands. TGFβ stimulation leads to phosphorylation and activation of SMAD1, SMAD2 and SMAD3, which form complexes with SMAD4 that accumulate in the nucleus and regulate transcription of target genes. SMAD signaling is negatively regulated by inhibitory SMADs and ubiquitin-mediated processes and proteasomal degradation of SMADs depend on the direct interaction of specific E3 ligases with SMADs. SMAD4 is targeted for degradation by multiple ubiquitin ligases that can simultaneously act on R-SMADs and signaling receptors. Such mechanisms of down-regulation of TGFβ signaling via degradation of SMADs may be critical for proper physiological response to this pathway.
外形
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
制备说明
after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥70% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| 分子量 | ~95 kDa |
| NCBI登记号 | NM_005359 |
| application(s) | cell analysis |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... SMAD4(4089) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
