Sphingosine kinase 1 Active human

产品说明

一般描述

Human Sphingosine kinase 1 (GenBank Accession No. AF200328), full length with N-terminal His tag, MW = 46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

单位定义

One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

产品性质