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SUMO Protease, Biotin tagged

品牌
Sigma-Aldrich
货号
SAE0101
规格纯度
Recombinant protein, aqueous solution, ≥25,000 units/mL
参考价格
5076.98 *本价格含增值税费
促销
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数量
-+
产品名称:
SUMO Protease, Biotin tagged
Small Ubiquitin-like Modifier Protease
ULP
Ubiquitin-homology domain protein PIC1
Ubiquitin like protease
Ubl-specific protease 1
产品介绍:

产品说明

一般描述

SUMO proteases are enzymes that specifically cleave the post-translational protein modification (PTM) known as small ubiquitin-related modifier (SUMO). SUMO falls into the PTM class of ubiquitin and/or ubiquitin-like proteins (UBL).SUMO protease is the Ubl-specific protease 1 (Ulp1) from Saccharomyces cerevisiae. This was the first of this class of enzymes to be isolated. SUMO protease cleaves specifically the SUMO moiety in a ‘scarless′ manner. After recognizing the tertiary structure of the Ubiquitin-like SUMO domain, SUMO protease hydrolyzes the peptide bond in the x–Gly–Gly–x sequence after the Gly-Gly bond, at the C-terminus of the SUMO domain. Besides the cleavage of natural SUMO-modified proteins, SUMO protease is used to cleave recombinant SUMO fusion proteins. The SUMO domain is a known solubility-enhancing fusion tag used in recombinant protein expression. Since this recombinant protease does not contain any coman protein purification tag it can be used for on-column cleavage of column bound SUMO fusion protein. Sumo protease with Biotin tag can be easily removed at the end of the digestion reaction.

应用

This biotin-tagged SUMO protease product is designed to be used for on-column cleavage of SUMO fusion proteins. This method specifically cleaves the protein of interest from a column-bound SUMO fusion protein, leaving the SUMO domain bound to the affinity column (e.g. Ni-NTA column) and eluting only the protein of interest. This method is advantageous to post-elution cleavage for several reasons:

Eliminates most of the impurities normally associated with purification on Ni-chelating columns.

  • Allows much gentler elution conditions, with an added flexibility in the composition of the elution buffer.
  • Assist preventing protein aggregation and inactivation.
  • Following cleavage, the protease can be efficiently removed by using any avidin-conjugated or streptavidin-conjugated beads.

This biotin-tagged SUMO protease has been enzymatically biotinylated without affecting its proteolytic activity. It does not include any additional protein purification tag (e.g., histidine-tag or GST).

单位定义

One enzyme unit is defined as the amount that will cut 90% of 100 pmol of SUMO-GST in 1 hour at 30℃.

产品性质

重组expressed in E. coli
测定≥90% (SDS-PAGE)
形式aqueous solution
分子量 27 kDa
浓度≥25,000 units/mL
运输dry ice
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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