产品说明
一般描述
T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.
应用
Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.
生化/生理作用
T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+ and Ca2+ activate this enzyme.
外形
Formulated in 200 mM imidazole and 20% glycerol.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | Escherichia coli |
| 重组 | expressed in E. coli |
| 测定 | ≥83% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 41.6 kDa |
| 包装 | pkg of 100 μg |
| NCBI登记号 | NP_049658 |
| UniProt登记号 | P04547 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | bacteriophage T4 ... B-GT(1258765) |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H319 - H360D |
| 预防措施声明 | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| 危险分类 | Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 |
| 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
