产品说明
一般描述
TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Recombinant human TIGAR expressed in E. coli is a 29.9 kDa protein containing 269 amino-acid residues.
外形
Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Tris, pH 8.0, 200 mM NaCl and 1 mM DTT.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥95% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 分子量 | 29.9 kDa |
| 包装 | pkg of 10 μg pkg of 50 μg |
| 杂质 | <1 EU/μg endotoxin (LAL test) |
| UniProt登记号 | Q9NQ88 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... TIGAR(57103) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H319 |
| 预防措施声明 | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| 危险分类 | Eye Irrit. 2 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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