产品说明
一般描述
TTK is a serine/threonine kinase that has been implicated in the regulation of centrosome duplication and mitotic checkpoint response. Overexpressing of dominant-negative TTK in human cell lines prevents centrosome duplication while active TTK accelerates centrosome reduplication in an osteosarcoma cell line. Disruption of TTK function leads to a combination of severe mitotic abnormalities and failure in centrosome duplication. TTK is required for stabilization and activation of p53 during spindle disruption. TTK phoshorylates the N-terminal domain of p53 at Thr18, and this phosphorylation disrupts the interaction with MDM2 and abrogates MDM2-mediated p53 ubiquitination.
外形
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
制备说明
after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.32 |
产品性质
| 重组 | expressed in baculovirus infected Sf9 cells |
| 产品线 | PRECISIO® Kinase |
| 测定 | ≥70% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| specific activity | 16-22 nmol/min·mg |
| 分子量 | ~130 kDa |
| NCBI登记号 | NM_003318 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... TTK(7272) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
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