产品说明
一般描述
Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 μM.
外形
Supplied at a concentration of 1.0 mg/ml in 20 mM TBS, pH 7.5 containing 1.0 mM DTT.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥95% (SDS-PAGE) |
| 形式 | liquid |
| 分子量 | 22.4 kDa |
| 包装 | pkg of 10 μg |
| 浓度 | ~1 mg/mL |
| 杂质 | Endotoxin, tested |
| NCBI登记号 | P61086 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... UBE2K(3093) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
