产品说明
一般描述
The Visfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Visfatin peptide based on the principle of Competitive Enzyme Immunoassay.
Visfatin, also known as nicotinamide phosphoribosyltransferase (Nampt)/ pre-B Cell colony enhancing factor is encoded by the gene mapped to human chromosome 7q22.3. The gene contains 11 exons encompassing 34.7kb. The encoded protein is an adipocytokine secreted by visceral fat.
免疫原
Visfatin, synthetic peptide
应用
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Visfatin EIA Kit has been used to measure the concentration of visfatin in blood samples.
生化/生理作用
Visfatin has a role in mammalian nicotinamide adenine dinucleotide (NAD+) biosynthesis. Studies have shown that mice deficient in visfatin in the forebrain excitatory neurons show hyperactivity, diminished anxiety-like behaviors and impaired learning and memory. It has also been shown that visfatin stimulates glucose uptake and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation in mouse C2C12 skeletal muscle cells. Visfatin regulates inflammation and insulin sensitivity.
基本信息
| NACRES | NA.32 |
产品性质
| species reactivity | mouse, rat, human |
| 包装 | kit of 96 wells (12 strips x 8 wells) |
| technique(s) | ELISA: suitable enzyme immunoassay: suitable |
| input | sample type plasma sample type culture supernatant(s) sample type cell lysate sample type serum |
| assay range | inter-assay cv: <15% intra-assay cv: <10% sensitivity: 0.778 ng/mL standard curve range: 0.1-1000 ng/mL |
| detection method | colorimetric |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... NAMPT(10135) |
组份列表
组份可单独销售
| 货号 | 组份 |
| RABHRP3 | HRP-Streptavidin |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H290 |
| 预防措施声明 | P234 - P390 |
| 危险分类 | Met. Corr. 1 |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 2 |
Sigma-Aldrich
m.cnreagent.com
