产品说明
一般描述
Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.
Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).
Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.
免疫原
Human
human peripheral blood monocytes
包装
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
Flow Cytometry (5-10 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Cell Inhibition Assays (see comments)
警告
Toxicity: Standard Handling (A)
外形
In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
重悬
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
分析说明
Negative Control
Nalm-6 cells
Positive Control
HPB-MLT cells
其他说明
Berman, M.E., et al. 1996. J. Immunol.156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161.
Kaneko, M., et al. 1995. J. Immunol.155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol.126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.
Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | IB4, monoclonal |
测定 | ≥95% (SDS-PAGE) |
形式 | liquid |
包含 | 0.5 mg/mL gentamicin sulfate as preservative |
species reactivity | human |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze |
同位素/亚型 | IgG2a |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich