Anti-Tau Mouse mAb (TAU-5)

产品说明

一般描述

This Anti-Tau Mouse mAb (TAU-5) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tau.
Recognizes the ~45-68 kDa Tau protein.
Protein G purified mouse monoclonal antibody. Recognizes the ~45-68 kDa phosphorylated and unphosphorylated forms of tau.

免疫原

Bovine
purified bovine microtubule-associated proteins
Epitope: within the central region

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (1-2 µg/ml)

Immunoblotting (1 µg/ml)

Immunoprecipitation (10 µg per 200-500 µg cell lysate)

Paraffin Sections (1-2 µg/ml, heat pre-treatment required)

警告

Toxicity: Standard Handling (A)

外形

In PBS.

重悬

Following initial thaw, aliquot and freeze (-70°C). Do not store diluted antibody without carrier protein if the concentration is <50 µg/ml.

其他说明

When used for formalin/paraffin embedded sections, staining is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min prior to antibody incubation. Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau. Does not cross-react with other MAPS of tubulin. Recognizes both native and phosphorylated forms of tau. Variables associated with assay conditions will dictate proper working dilution.
Papasozomenos, S.C. and Shanavas, A., 2002. Proc. Natl. Acad. Sci. USA99, 1140.
Rapoport, M., eta al. 2002. Proc. Natl. Acad. Sci. USA99, 6364.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息