产品说明
一般描述
Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.
Note: 1 mU = 1 milliunit.
包装
500 miu in Plastic ampoule
应用
Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
警告
Toxicity: Harmful (C)
单位定义
One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.
外形
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
其他说明
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 酶学委员会(EC)编号 | 3.2.1.18 ( BRENDA | IUBMB ) |
产品性质
| 质量水平 | 100 |
| 重组 | expressed in E. coli |
| 产品线 | EMPROVE® EXPERT |
| 形式 | liquid |
| specific activity | ≥10 units/mL ≥250 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | do not freeze |
| 异质活性 | N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |   |
| 警示用语： | Warning |
| 危险声明 | H290 - H315 - H319 - H335 |
| 预防措施声明 | P234 - P261 - P264 - P271 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 8B - Non-combustible, corrosive hazardous materials |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
