Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1)

产品说明

一般描述

Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing spleonocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the c-ErbB2/c-neu protein.
Anti-c-ErbB2/c-Neu (Ab-5), mouse monoclonal, clone TA-, recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells. It is validated for FC, WB, IF, IP, and IHC on frozen sections.

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Flow Cytometry (5 µg/ml, see application references)

Frozen Sections (5 µg/ml)

Immunoblotting (see application references)

Immunofluorescence (5 µg/ml, see application references)

Immunoprecipitation (1 µg/sample, see application references)

Neutralization Studies (not recommended, see comments and application references)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM PBS, 0.2% gelatin, pH 7.5.

分析说明

Positive Control
SK-BR-3 cells

其他说明

Recognizes an extracellular epitope and does not cross react with human EGF-receptor. It will not inhibit in vivo protein tyrosine kinase activity. Antibody should be titrated for optimal results in individual systems.
DiFiore, P.P., et al. 1987. Science237, 178.
Slamon, D.J., et al. 1987. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984 Cold Spring Harbor Laboratory2, 215.
Bargmann, C.I., et al. 1968. Nature319, 226.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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