Anti-CD64 Mouse mAb (10.1)

产品说明

一般描述

Recognizes the ~72 kDa CD64 protein (FcR1 receptor).
Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.
Purified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein.

免疫原

human rheumatoid synovial fluid cells/monocytes
Human

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Flow Cytometry (10 µg/ml)

Frozen Sections (see comments)

Immunoblotting (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

重悬

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

分析说明

Negative Control
HPB-MLT
Positive Control
4937 cells

其他说明

The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x10⁵ cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Dougherty, G.J., et al. 1987. Eur. J. Immunol.17, 1453.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息