Anti-CD44 Rat mAb (A020)

产品说明

一般描述

Rat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.
Recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.
Anti-CD44, rat monoclonal, clone A020, recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 in mouse. It is validated for ELISA, IF, IP, RIA, WB, and IHC.

免疫原

purified human lymphocyte CD44
Human

包装

100 μL in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

ELISA (1:2000)

Flow Cytometry (1:1000)

Immunoblotting (1:1000)

Immunofluorescence (1:200)

Immunohistochemistry (1:250)

Immunoprecipitation (1:200)

Neutralization Studies (see comments and application references)

Radioimmunoassay (1:5000)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-20°C).

其他说明

The antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.
Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol.162, 121.
Bourguignon, L.Y.W., et al. 1993. J. Immunol.151, 6634.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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