产品说明
一般描述
Recognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and colon tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP20 mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.
This Anti-MSH2 (Ab-1) Mouse mAb (GB12) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of MSH2 (Ab-1).
免疫原
Human
an N-terminal fragment of human MSH2
包装
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
Frozen Sections (5 µg/ml)
Immunoblotting (1 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (3 µg/ml, heat pre-treatment required)
警告
Toxicity: Standard Handling (A)
外形
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
分析说明
Positive Control
HCT116 or SW480 cells or colon tissue
Negative Control
LoVo cells
其他说明
Bronner, C. E., et al. 1994. Nature368, 258.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P. T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027-1038, 1993.
Leach, F. S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
For paraffin sections, pre-treat with heat for 20 min. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | GB12, monoclonal |
形式 | liquid |
包含 | ≤0.1% sodium azide as preservative |
species reactivity | human |
should not react with | mouse |
manufacturer/tradename | Calbiochem® |
储存条件 | do not freeze |
同位素/亚型 | IgG1 |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich