Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801)

产品说明

一般描述

Recognizes the ~53 kDa wild-type and mutant p53 protein in A-431 cells and breast carcinoma tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
This Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IP, Paraffin Sections for the detection of p53 (Ab-2) (Pantropic).

免疫原

a human p53 fusion protein
Epitope: within amino acids 46-55 of human p53
Human

包装

100, 200 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (5 µg/ml)

Gel Shift (see comments)

Immunoblotting (2.5 µg/ml)

Immunoprecipitation (1 µg/sample)

Paraffin Sections (5 µg/ml, pepsin, trypsin, or heat pre-treatment required)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Positive Control
A431 cells or breast carcinoma tissue
Negative Control
SK-OV-3 cells or normal skin

其他说明

Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53; short incubation times with ³⁵S-Met (≤ 1 h) will help reduce background. For immunoblots of wild-type p53, maximize sensitivity by preconcentrating samples by immunoprecipitation with Cat. No. OP09, then immunoblot using Cat. No. PC35 and chemiluminescent detection. For a gel shift assay, use Cat. No. OP09L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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