Anti-p300 Mouse mAb (NM-11)

产品说明

一般描述

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.
Recognizes the p300 protein in A431, HeLa, and ML-1 cells.
This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.

免疫原

Epitope: within amino acids 2071-2091 near the CBP C-terminus
recombinant, human p300 protein
Human

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1 µg/sample)

Paraffin Sections (1-5 µg/ml, heat pre-treatment required)

警告

Toxicity: Standard Handling (A)

外形

In 0.05 M sodium phosphate buffer, 50% glycerol.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
A431, HeLa, or ML-1 cells

其他说明

Dallas, P.B., et al. 1997. Hybridoma16, 273.
Shisler, J., et al. 1996. J. Virol.70, 68.
Wang, H.G., et al. 1995. J. Virol.69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene9, 1733.
Eckner, R., et al. 1994. Genes Dev.8, 869.
Abraham, S.E., et al. 1993. Oncogene8, 1639.
Wang, H.G., et al. 1993. J. Virol.67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol.11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol.11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol.8, 3955.
Yee, S. and Branton, P., 1985. Virology147, 142.
Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息