Anti-PCNA (Ab-1) Mouse mAb (PC10)

产品说明

一般描述

Recognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1).

免疫原

Epitope: Within amino acids 112-121
recombinant PCNA

包装

200 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (2.5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (not recommended with unconjugated antibody, see comments)

Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)

警告

Toxicity: Standard Handling (A)

分析说明

Positive Control
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue

其他说明

Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci.96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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