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Anti-Ubiquitin Mouse mAb (FK2)

泛素小鼠单克隆抗体(FK2)

品牌
Sigma-Aldrich
货号
ST1200
规格纯度
liquid, clone FK2, Calbiochem®
参考价格
5854.74 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Anti-Ubiquitin Mouse mAb (FK2)
泛素小鼠单克隆抗体(FK2)
产品介绍:

产品说明

一般描述

This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.
Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins.

免疫原

unpurified polyubiquitinylated lysozyme
human

包装

100 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

应用


ELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)

警告

Toxicity: Standard Handling (A)

外形

In PBS.

制备说明

Raji cells

重悬

Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.

其他说明

Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式unpurified
antibody product typeprimary antibodies
克隆FK2, monoclonal
形式liquid
包含≤0.1% sodium azide as preservative
species reactivity (predicted by homology)all
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型IgG1
运输wet ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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