( S )-乳酸盐：NAD + 氧化还原酶

产品名称：

9001-60-9
Lactic Dehydrogenase, recombinant from E. coli
(S)-Lactate: NAD⁺ oxidoreductase
Lactate
L-Lactate Dehydrogenase
乳酸脱氢酶，重组
( S )-乳酸盐：NAD ⁺ 氧化还原酶
乳酸
L- 乳酸脱氢酶

产品介绍：

产品说明

一般描述

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.

应用

Lactic Dehydrogenase, recombinant from E. coli has been used:

in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
in in vitro DltC D-alanylation assay

生化/生理作用

Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.
L -乳酸脱氢酶催化 L -乳酸转化为 L -丙酮酸，同时还原 NAD ⁺ 为 NADH 和 H ⁺。

单位定义

One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25℃ (NADH as cofactor)

基本信息

酶学委员会(EC)编号	1.1.1.27 ( BRENDA \| IUBMB )
EC 号	232-617-8
MDL编号	MFCD00131466
NACRES	NA.54

产品性质

质量水平	100
重组	expressed in E. coli
形式	powder
specific activity	≥90 U/mg
储存温度	−20℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)

Lactic Dehydrogenase, recombinant from E. coli

乳酸